Yong Doo Park, Nicola Tirelli, Jeffrey A. Hubbell
Department of Materials, Institute for Biomedical Engineering, Swiss Federal Institute of Technology and University of Zurich, Moussonstrasse 18, CH-8044 Zurich, Switzerland
Received 3 May 2002; Accepted 6 September 2002. Available online 19 December 2002.
Corresponding author : Nicola Tirelli ; Tel.: +41-1-632-63-48; fax: +41-1-632-12-14
Hyaluronic acid (HA) was derivatized with methacrylic esters used for the preparation of hydrogels via photopolymerization. Poly(ethylene glycol) diacrylate (PEG-DA) with a molecular weight of 570 was also used as a comacromonomer to improve elastic modulus and swelling behavior. The hydrogels were readily degraded by hyaluronidase and their mechanical properties could be modulated by HA molecular weight and concentration of PEG-DA. The incorporation of RGD peptides allowed modulation of the HA properties from cell non-adhesive to adhesive. Human dermal fibroblasts were cultured on the RGD, RDG, and non-functionalized HA hydrogels for up to 7 d, showing adhesion and proliferation only with incorporated RGD.
Keywords: Hyaluronic acid; Hydrogels; Photopolymerization; RGD; Cell adhesion